Composite
Part:BBa_K4724085:Design
Designed by: Xinyao Li Group: iGEM23_NJTech-China-A (2023-10-10)
TrxA-LSPETase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1192
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 322
Illegal NgoMIV site found at 448
Illegal AgeI site found at 535 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The DNA sequences of LSPETase and TrxA were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of the TrxA tag at the N-terminus of LSPETase.
Source
Laboratory design in our research group.